biochemical and morphological changes in bone marrow mesenchymal stem cells induced by treatment of rats with p-nonylphenol

نویسندگان

mohammad hossein abnosi biology department, faculty of sciences, arak university, arak, iran

elham shojafar biology department, faculty of sciences, arak university, arak, iran

چکیده

objective(s):in previous investigations, we have shown para-nonylphenol (p-np) caused significant reduction of proliferation and differentiation of rat bone marrow mesenchymal stem cells (mscs) in vitro. in this study, we first treat the rats with p-np, then carried out the biochemical and morphological studies on mscs. materials and methods: proliferation property of cells was evaluated with the help of mtt assay, trypan blue, population doubling number, and colony forming assay. differentiation property was evaluated with quantitative alizarin red assay, measurement of alkaline phosphatase (alp) activity as well as intracellular calcium content. in addition; morphological study, tunel test, activated caspase assay, and comet assay were performed to evaluate the mechanism of the cell death. results: the results showed significant reduction in the colony-forming-ability and population-doubling-number of extracted cells when compared to control ones. in addition, it was revealed that the p-np treatment of rats caused significant reduction in nuclear diameter, cytoplasm shrinkage, and induction of caspase-dependent-apoptosis. also there was significant reduction in alp activity, intracellular calcium content, and intracellular matrix following osteogenic differentiation. conclusion: as mscs are the cellular back up for bone remodeling and repair, we suggest more investigations to be conducted regarding the correlation between the increasing number of patients suffering from osteoporosis and p-np toxicity. also, we strongly recommend who and local health organization to prevent industries of using p-np in formulation of industrial products which may cause changes in proliferation and differentiation properties of stem cells.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Biochemical and morphological changes in bone marrow mesenchymal stem cells induced by treatment of rats with p-Nonylphenol

Objective(s):In previous investigations, we have shown para-nonylphenol (p-NP) caused significant reduction of proliferation and differentiation of rat bone marrow mesenchymal stem cells (MSCs) in vitro. In this study, we first treat the rats with p-NP, then carried out the biochemical and morphological studies on MSCs. Materials and Methods: Proliferation property of cells was evaluated with t...

متن کامل

Biochemical and morphological changes in bone marrow mesenchymal stem cells induced by treatment of rats with p-Nonylphenol

OBJECTIVES In previous investigations, we have shown para-nonylphenol (p-NP) caused significant reduction of proliferation and differentiation of rat bone marrow mesenchymal stem cells (MSCs) in vitro. In this study, we first treat the rats with p-NP, then carried out the biochemical and morphological studies on MSCs. MATERIALS AND METHODS Proliferation property of cells was evaluated with th...

متن کامل

the induction of apoptosis and autophagy in rats bone marrow mesenchymal stem cells following in vitro treatment with p-nonylphenol

p-nonylphenol (p-np) as a non ionic surfactant is used in many industries such as pesticides, cosmetics,detergents and so on. our previous study showed that the p-np causes reduction in viability of the rats bonemarrow mesenchymal stem cells (mscs). the aim of the present study was to investigate the mechanism of the cell death due to p-np exposure. the cells, after 3rd passage were treated wit...

متن کامل

Research Paper: Investigating Morphologic Changes and Viability of Rats’ Bone Marrow Mesenchymal Stem Cells in Microgravity

Introduction: Mesenchymal Stem Cells (MSCs) are multipotent cells capable of duplication and auto-recovery and distinction from various cells including chondrocytes, adipocytes, chondroblasts, fibroblasts, and osteoblasts. Human stem cells are always subject to local and external mechanical loads. External loads are caused by physical activity in external environment loading to infliction of st...

متن کامل

Harvesting of bone marrow mesenchymal stem cells from live rats and the in vitro differentiation of bone marrow mesenchymal stem cells into neuron-like cells

In the bone marrow, there are certain populations of stem cell sources with the capacity to differentiate into several different types of cells. Ideally, cell transplants would be readily obtainable, easy to expand and bank, and capable of surviving for sufficient periods of time. Bone marrow mesenchymal stem cells (BM-MSCs) possess all of these characteristics. One of the most important benefi...

متن کامل

Regenerative Effect of Bone Marrow-derived Mesenchymal Stem Cells in Thioacetamide-induced Liver Fibrosis of Rats

The present study determined the regenerative effect of bone marrow-derived stem cells (BMSCs) on thioacetamide (TA)-induced liver fibrosis in rats. A total of 30 male Wistar rats were randomly divided into sham control and treatment groups. The rats of the sham control group were subdivided into three groups and sampled on the 14th, 18th, and 20th weeks after fibrosis induction. The rats of th...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۸، شماره ۴، صفحات ۳۱۷-۳۲۴

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023